The detection of antibodies cross-reacting with Cowdria ruminantium in the sera of domestic ruminants in regions of South Africa where Amblyomma hebraeum does not occur.
نویسندگان
چکیده
High levels of seropositivity, in all probability attributable to Ehrlichia, were recorded in the serum of domestic ruminants throughout districts in South Africa where Amblyomma hebraeum, the vector of the heartwater agent, does not occur. The antibodies, detected with the indirect fluorescent antibody (IFA) and the indirect ELISA tests, cross-reacted with Cowdria ruminantium, which was used as antigen in both tests. A combination of the IFA and ELISA tests, currently employed to detect antibodies to C. ruminantium, facilitates the handling of appreciable numbers of sera and ensures maximum reliability.
منابع مشابه
The prevalence of Cowdria ruminantium in free-living adult Amblyomma hebraeum collected at a communal grazing area and in 2 wildlife reserves in South Africa.
In order to detect the prevalence of Cowdria ruminantium in the vector tick, Amblyomma hebraeum, free-living, unfed adult ticks were collected with the aid of pheromone/CO2 traps. Ticks were collected at the Rietgat communal grazing area, as well as in the southwestern Kruger National Park and in the Songimvelo Game Reserve, all located in heartwater-endemic areas of South Africa. The presence ...
متن کاملThe detection of antibodies to Cowdria ruminantium in serum and C. ruminantium antigen in Amblyomma hebraeum by an enzyme-linked immunosorbent assay.
A sensitive and reliable enzyme-linked immunosorbent assay for the detection of antibodies to Cowdria ruminantium in serum and C. ruminantium antigen in Amblyomma hebraeum nymphae is described. For the screening of antibodies, C. ruminantium from A. hebraeum nymphae, partially purified by wheat-germ lectin affinity chromatography, was used as antigen. To screen nymph populations, sera from eith...
متن کاملHeartwater. The artificial transmission of Cowdria ruminantium in domestic ruminants and mice.
The artificial transmission of Cowdria ruminantium with infected blood, organ homogenates, peritoneal macrophages, tick stabilate and tissue culture cells is discussed. Organ homogenates prepared from the myocardium, spleen, kidneys and liver of diseased animals are commonly used to infect mice. The efficacy of organ homogenates as a source of C. ruminantium depends on factors such as the route...
متن کاملDetection of the agent of heartwater, Cowdria ruminantium, in Amblyomma ticks by PCR: validation and application of the assay to field ticks.
We have previously reported that the pCS20 PCR detection assay for Cowdria ruminantium, the causative agent of heartwater disease of ruminants, is more sensitive than xenodiagnosis and the pCS20 DNA probe for the detection of infection in the vector Amblyomma ticks. Here, we further assessed the reliability of the PCR assay and applied it to field ticks. The assay detected DNA of 37 isolates of...
متن کاملA method for determining the Cowdria ruminantium infection rate of Amblyomma hebraeum: effects in mice injected with tick homogenates.
Amblyomma hebraeum ticks, collected in the field and individually homogenized, were injected into mice. Thirteen out of 240 ticks were shown to be infected with the heartwater agent. Antibodies against Cowdria ruminantium were detected in the sera of the mice by means of the indirect fluorescent antibody test. Giemsa-stained smears, prepared from the haemocytes of the ticks, revealed morphologi...
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ورودعنوان ژورنال:
- The Onderstepoort journal of veterinary research
دوره 61 4 شماره
صفحات -
تاریخ انتشار 1994